fplc (Amersham Life Sciences Inc)
86
Structured Review
Amersham Life Sciences Inc
fplc
Fplc, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fplc+system/fplc/10__36721_slash_pjps__2026__39__7__201__1-59-22-23
Average 86 stars, based on 1 article reviews
Fplc, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fplc+system/fplc/10__36721_slash_pjps__2026__39__7__201__1-59-22-23
Average 86 stars, based on 1 article reviews
fplc - by Bioz Stars,
2026-09
86/100 stars
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Purification:Article Title: Glutaminolysis and Transferrin Regulate Ferroptosis Article Snippet: .. Purification and identification of transferrin from FBS All purification steps were carried out at 4°C, and chromatography was performed with an Article Title: Isoniazid resistance without a loss of fitness in Mycobacterium tuberculosis Article Snippet: .. The lysate was applied to a Histrap FF crude column (GE Healthcare) and purified using an Article Title: Decoupling mutations in the D-channel of the aa 3 -type cytochrome c oxidase from Rhodobacter sphaeroides suggest that a continuous hydrogen-bonded chain of waters is essential for proton pumping Article Snippet: UV/Vis spectroscopy UV/Vis spectra were recorded with a Shimadzu UV/Vis-2101PC spectrophotometer. .. 5-10 mg of purified protein were diluted to 4 ml with 10 mM Tris-HCl buffer, pH 8.0, containing 1mM EDTA and 0.05% DDM (buffer A), and loaded onto a DEAE-5PW column (Toso-Haas) attached to an Chromatography:Article Title: Glutaminolysis and Transferrin Regulate Ferroptosis Article Snippet: .. Purification and identification of transferrin from FBS All purification steps were carried out at 4°C, and chromatography was performed with an Fast Protein Liquid Chromatography:Article Title: Glutaminolysis and Transferrin Regulate Ferroptosis Article Snippet: .. Purification and identification of transferrin from FBS All purification steps were carried out at 4°C, and chromatography was performed with an Article Title: Role of protons in the pump cycle of KdpFABC investigated by time-resolved kinetic experiments. Article Snippet: The time-resolved kinetics of the KdpFABC complex solubilized in Aminoxide WS-35 was investigated by ATP concentration jump experiments.. ATP was photoreleased from its inactive precursor, caged ATP, and charge movements in the membrane domain of the KdpFABC were detected by the electrochromic dye RH421.. At low ATP concentrations, the ATP binding step became rate-limiting with an apparent, pH-independent ATP binding affinity of ∼70 μM. Article Title: The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues Article Snippet: .. The generated AGEs were separated on HW-40S column (1.6 × 100 cm, Toyopearl, Tosoh) in the 0.1 M acetate buffer, pH 6.8 using the Article Title: Mechanistic analysis of the pump cycle of the KdpFABC P-type ATPase. Article Snippet: The solubilized protein was collected by centrifugation at 200,000×g at 4 °C for 60 minutes, and the supernatant was applied to a 5 mL HisTrap column (GE Healthcare), pre-equilibrated with 50 mM Tris-HCl, 20 mM MgCl2, 10% (v/v) glycerol, 150 mM NaCl, 10 mM imidazole, pH 7.5, 0.5 mM PMSF and 0.2% (w/v) Aminoxide WS-35. .. After 3 binding cycles, the HisTrap column was connected to a Article Title: Cloning and biochemical characterization of Staphylococcus aureus type IA DNA topoisomerase comprised of distinct five domains. Article Snippet: DNA topoisomerases play critical roles in regulating DNA topology and are essential enzymes for cell survival.. In this study, a gene encoding type IA DNA topoisomerase was cloned from Staphylococcus aureus (S. aureus) sp. strain C-66, and the biochemical properties of recombinant enzyme was characterized.. The nucleotide sequence analysis showed that the cloned gene contained an open reading frame (2070 bp) that could encode a polypeptide of 689 amino acids. Article Title: Decoupling mutations in the D-channel of the aa 3 -type cytochrome c oxidase from Rhodobacter sphaeroides suggest that a continuous hydrogen-bonded chain of waters is essential for proton pumping Article Snippet: UV/Vis spectroscopy UV/Vis spectra were recorded with a Shimadzu UV/Vis-2101PC spectrophotometer. .. 5-10 mg of purified protein were diluted to 4 ml with 10 mM Tris-HCl buffer, pH 8.0, containing 1mM EDTA and 0.05% DDM (buffer A), and loaded onto a DEAE-5PW column (Toso-Haas) attached to an Generated:Article Title: The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues Article Snippet: .. The generated AGEs were separated on HW-40S column (1.6 × 100 cm, Toyopearl, Tosoh) in the 0.1 M acetate buffer, pH 6.8 using the Binding Assay:Article Title: Mechanistic analysis of the pump cycle of the KdpFABC P-type ATPase. Article Snippet: The solubilized protein was collected by centrifugation at 200,000×g at 4 °C for 60 minutes, and the supernatant was applied to a 5 mL HisTrap column (GE Healthcare), pre-equilibrated with 50 mM Tris-HCl, 20 mM MgCl2, 10% (v/v) glycerol, 150 mM NaCl, 10 mM imidazole, pH 7.5, 0.5 mM PMSF and 0.2% (w/v) Aminoxide WS-35. .. After 3 binding cycles, the HisTrap column was connected to a other:Article Title: Several posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission Article Snippet: The clear cell lysate was collected by |